Wang H B, Zheng Q Y, Chen H S
Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai, China.
Zhongguo Yao Li Xue Bao. 1993 Nov;14 Suppl:S30-3.
Phytolacca acinose polysaccharides I (PAP-I), M(r) = 10,000, activated mouse splenocytes to produce colony-stimulating factors (CSF) in vitro. The level of CSF was tested by [3H]TdR uptaken by bone marrow cells and rmGM-CSF was used as standard. PAP-I (10-500 micrograms.ml-1) increased CSF production of the splenocytes treated with or without concanavalin A. When the concentration of PAP-I was 100 micrograms.ml-1, the level of CSF was about equivalent to that of rmGM-CSF 11.8 +/- 1.8 ng.ml-1. After a 3-d incubation of PAP-I with the splenocytes, CSF was assayed. The longer the incubation, the higher were the CSF concentrations. The CSF type in supernants of splenocytes induced by PAP-I was determined by IL-3 McAb, GM-CSF McAb, and M-CSF PcAb. The type of CSF was found to be interleukin-3.
商陆多糖I（PAP-I），相对分子质量为10,000，在体外可激活小鼠脾细胞产生集落刺激因子（CSF）。通过骨髓细胞摄取的[3H]TdR检测CSF水平，并以重组小鼠粒细胞-巨噬细胞集落刺激因子（rmGM-CSF）作为标准。PAP-I（10 - 500微克·毫升-1）可增加经或未经伴刀豆球蛋白A处理的脾细胞的CSF产生。当PAP-I浓度为100微克·毫升-1时，CSF水平约相当于11.8±1.8纳克·毫升-1的rmGM-CSF。PAP-I与脾细胞孵育3天后，检测CSF。孵育时间越长，CSF浓度越高。用白细胞介素-3单克隆抗体（IL-3 McAb）、粒细胞-巨噬细胞集落刺激因子单克隆抗体（GM-CSF McAb）和巨噬细胞集落刺激因子多克隆抗体（M-CSF PcAb）测定PAP-I诱导的脾细胞上清液中的CSF类型。发现CSF类型为白细胞介素-3。
