Chiappelli F, Kung M, Lee P, Pham L, Manfrini E, Villanueva P
Laboratory of Human Oral and Molecular Immunology, University of California-Los Angeles School of Dentistry 90095-1668, USA.
Alcohol Clin Exp Res. 1995 Jun;19(3):539-44. doi: 10.1111/j.1530-0277.1995.tb01545.x.
We are interested in the characterization of the effects of alcohol on human T-cell activation, maturation, and migration, because this cell population is crucial in the initiation, regulation, and propagation of cellular immunity. We and others have described the effects of both acute and chronic exposure of human immune cells to ethanol (EtOH) in vitro. Herein, we briefly, review these reports and expand this body of literature with the inclusion of new data recently obtained in our laboratory. We confirm the blunting effects of EtOH on the production of interleukin-2 and mitogen proliferative response following T-cell mitogen stimulation, and on the expression of membrane markers of activation. We show that EtOH significantly alters the expression of the CD4 cell-associated marker of activation, CD26. We report the effect of EtOH on the expression of the homing receptor CD62L by CD4+ cells, and on their ability to adhere by a CD18-mediated process to a defined cellular substratum. Furthermore, we demonstrate the effects of EtOH and EtOH and beta-endorphin pretreatment on the activation of CD4+ lymphocytes endowed with the homing receptor CD62L.
我们对酒精对人T细胞激活、成熟和迁移的影响特征感兴趣，因为这个细胞群体在细胞免疫的启动、调节和传播中至关重要。我们和其他人已经描述了人免疫细胞在体外急性和慢性暴露于乙醇（EtOH）的影响。在此，我们简要回顾这些报告，并通过纳入我们实验室最近获得的新数据来扩展这一文献体系。我们证实了乙醇对T细胞有丝分裂原刺激后白细胞介素-2产生和有丝分裂原增殖反应以及激活膜标志物表达的抑制作用。我们表明乙醇显著改变了与CD4细胞相关的激活标志物CD26的表达。我们报告了乙醇对CD4+细胞归巢受体CD62L表达的影响，以及它们通过CD18介导的过程黏附到特定细胞基质上的能力。此外，我们证明了乙醇以及乙醇和β-内啡肽预处理对具有归巢受体CD62L的CD4+淋巴细胞激活的影响。
