Pontet F, Later R, Manceaux J C, Pressac M, Boigné J M
Service de biochimie, hôpital Lariboisière, Paris, France.
Ann Biol Clin (Paris). 1995;53(5):283-91.
This study is the third part of a multicenter evaluation carried out with ten analysers. Five proteins (IgG, IgA, IgM, transferrin, haptoglobin) were assayed in three sera, each containing one monoclonal Ig (IgG, IgA, or IgM). The expected agreement was not obtained with these particular sera, except in the case of haptoglobin. The marked imprecision of the monoclonal Ig quantification is highlighted, including the fact that many erroneous results are due to the antigen excess phenomenon. It is also shown that in the serum containing monoclonal IgM, quantification of the other proteins, remaining polyclonal Ig, as well as transferrin and haptoglobin, is difficult. The authors recall that electrophoresis and immunoelectrophoresis, which are indispensable for the characterization of the monoclonal components, are of great help in solving such difficulties.
本研究是使用十台分析仪进行的多中心评估的第三部分。在三种血清中检测了五种蛋白质（IgG、IgA、IgM、转铁蛋白、触珠蛋白），每种血清含有一种单克隆Ig（IgG、IgA或IgM）。除了触珠蛋白外，使用这些特定血清未获得预期的一致性。突出显示了单克隆Ig定量的明显不精密度，包括许多错误结果是由于抗原过量现象这一事实。还表明，在含有单克隆IgM的血清中，对其他蛋白质（剩余的多克隆Ig）以及转铁蛋白和触珠蛋白进行定量很困难。作者提醒，电泳和免疫电泳对于单克隆成分的表征是不可或缺的，在解决此类困难方面有很大帮助。
