Restaino L, Frampton E W, Hemphill J B, Palnikar P
R & F Laboratories, Inc., Bridgeview, Illinois 60455, USA.
Appl Environ Microbiol. 1995 Sep;61(9):3471-5. doi: 10.1128/aem.61.9.3471-3475.1995.
The antimicrobial effects of ozonated water in a recirculating concurrent reactor were evaluated against four gram-positive and four gram-negative bacteria, two yeasts, and spores of Aspergillus niger. More than 5 log units each of Salmonella typhimurium and Escherichia coli cells were killed instantaneously in ozonated water with or without addition of 20 ppm of soluble starch (SS). In ozonated water, death rates among the gram-negative bacteria--S. typhimurium, E. coli, Pseudomonas aeruginosa, and Yersinia enterocolitica--were not significantly different (P > 0.05). Among gram-positive bacteria, Listeria monocytogenes was significantly P < 0.05) more sensitive than either Staphylococcus aureus or Enterococcus faecalis. In the presence of organic material, death rates of S. aureus compared with L. monocytogenes and E. coli compared with S. typhimurium in ozonated water were not significantly (P > 0.05) affected by SS addition but were significantly reduced (P < 0.05) by addition of 20 ppm of bovine serum albumin (BSA). More than 4.5 log units each of Candida albicans and Zygosaccharomyces bailii cells were killed instantaneously in ozonated water, whereas less than 1 log unit of Aspergillus niger spores was killed after a 5-min exposure. The average ozone output levels in the deionized water (0.188 mg/ml) or water with SS (0.198 mg/ml) did not differ significantly (P < 0.05) but were significantly lower in water containing BSA (0.149 mg/ml).
在循环式并行反应器中评估了臭氧水对四种革兰氏阳性菌、四种革兰氏阴性菌、两种酵母菌以及黑曲霉孢子的抗菌效果。无论是否添加20 ppm的可溶性淀粉（SS），鼠伤寒沙门氏菌和大肠杆菌细胞在臭氧水中均能瞬间被杀死超过5个对数单位。在臭氧水中，革兰氏阴性菌——鼠伤寒沙门氏菌、大肠杆菌、铜绿假单胞菌和小肠结肠炎耶尔森氏菌——的死亡率没有显著差异（P>0.05）。在革兰氏阳性菌中，单核细胞增生李斯特菌比金黄色葡萄球菌或粪肠球菌更敏感（P<0.05）。在有有机物质存在的情况下，臭氧水中金黄色葡萄球菌与单核细胞增生李斯特菌的死亡率以及大肠杆菌与鼠伤寒沙门氏菌的死亡率相比，添加SS对其没有显著影响（P>0.05），但添加20 ppm的牛血清白蛋白（BSA）会使其显著降低（P<0.05）。白色念珠菌和拜耳接合酵母细胞在臭氧水中均能瞬间被杀死超过4.5个对数单位，而黑曲霉孢子在暴露5分钟后被杀死的数量不到1个对数单位。去离子水（0.188 mg/ml）或含SS的水（0.198 mg/ml）中的平均臭氧输出水平没有显著差异（P<0.05），但含BSA的水中的平均臭氧输出水平显著较低（0.149 mg/ml）。
