Henderson J S, Johnson R B
Department of Periodontics, University of Mississippi Medical Center, Jackson 39216-4505, USA.
Arch Oral Biol. 1995 Jul;40(7):615-21. doi: 10.1016/0003-9969(95)00022-h.
Short-term exposure to smokeless tobacco extracts (STE) reportedly inhibits osteoblast metabolism. The objective of this study was to determine the effects of serial dilutions of a water-soluble extract of smokeless tobacco on osteoblast proliferation and their potential to form and mineralize bone nodules. STE significantly stimulated cell proliferation when diluted 10(2)-10(4) times; 10(3) and 10(4) dilutions produced the greatest effect. 10(2)-10(4) STE dilutions significantly increased alkaline phosphatase activity at day 7 but 10(6) STE dilutions significantly decreased it. 10(3) and 10(4) dilutions significantly increased bone nodule formation, but inhibited their mineralization. In contrast, 10(5) and 10(6) dilutions significantly decreased bone nodule formation, but increased their mineralization. Stimulation of in vitro bone nodule formation by STE was similar to that produced by 10(-7) M insulin-like growth factor 1 (IGF-1) in vivo. Heat and acid treatment of STE significantly reduced its beneficial effect on cell proliferation, suggesting that a peptide within STE may be responsible for enhancement of osteogenic cell proliferation. Thus, STE may contain a peptide capable of significantly stimulating osteoblast proliferation, differentiation and metabolism, similar to the effects of IGF-1. This peptide could have potential therapeutic benefits.
据报道，短期接触无烟烟草提取物（STE）会抑制成骨细胞代谢。本研究的目的是确定无烟烟草水溶性提取物的系列稀释液对成骨细胞增殖及其形成和矿化骨结节潜力的影响。当稀释10² - 10⁴倍时，STE显著刺激细胞增殖；10³和10⁴稀释度产生的效果最大。10² - 10⁴倍的STE稀释液在第7天显著增加碱性磷酸酶活性，但10⁶倍的STE稀释液则使其显著降低。10³和10⁴稀释度显著增加骨结节形成，但抑制其矿化。相比之下，10⁵和10⁶稀释度显著减少骨结节形成，但增加其矿化。STE对体外骨结节形成的刺激作用与体内10⁻⁷ M胰岛素样生长因子1（IGF-1）产生的作用相似。对STE进行加热和酸处理显著降低了其对细胞增殖的有益作用，这表明STE中的一种肽可能是成骨细胞增殖增强的原因。因此，STE可能含有一种能够显著刺激成骨细胞增殖、分化和代谢的肽，其作用类似于IGF-1。这种肽可能具有潜在的治疗益处。
