Visconti L A, Yen E H, Johnson R B
Department of Preventive Dental Science, University of Manitoba, Winnipeg, Canada.
Arch Oral Biol. 1998 Sep;43(9):729-33. doi: 10.1016/s0003-9969(98)00027-2.
Sodium acetate reportedly promotes bone atrophy by inducing resorption and inhibiting osteoprogenitor-cell proliferation, but little is known about its effects on bone-matrix deposition and mineralization by a population containing osteoprogenitor cells. The objective here was to assess the effects of 1-20 mM sodium acetate on the proliferation and differentiation of these cells and their resultant bone-nodule formation and mineralization in an in vitro assay. Exposure to 10 mM sodium acetate had no effect on cellular proliferation but significantly increased the production and mineralization of bone nodules (p < 0.01), suggesting that it affected osteoprogenitor differentiation and subsequent metabolism. However, 10 mM acetate did not increase net bone mass. Dilutions of 1-5 and 20 mM inhibited cellular proliferation and resultant bone-nodule formation and mineralization, significantly reducing the percentage bone area as compared to controls (p < 0.001). These data suggest that 1-5 and 20 mM sodium acetate significantly inhibit bone deposition, whereas 10 mM has no effects, which could contribute to iatrogenic metabolic bone disease in patients receiving either renal dialysis or total parenteral nutrition.
据报道，醋酸钠通过诱导骨吸收和抑制成骨祖细胞增殖来促进骨质流失，但对于其对含有成骨祖细胞群体的骨基质沉积和矿化的影响却知之甚少。本研究的目的是在体外试验中评估1-20 mM醋酸钠对这些细胞增殖和分化以及由此产生的骨结节形成和矿化的影响。暴露于10 mM醋酸钠对细胞增殖没有影响，但显著增加了骨结节的产生和矿化（p < 0.01），表明它影响了成骨祖细胞的分化和随后的代谢。然而，10 mM醋酸钠并没有增加净骨量。1-5 mM和20 mM的稀释液抑制细胞增殖以及由此产生的骨结节形成和矿化，与对照组相比，显著降低了骨面积百分比（p < 0.001）。这些数据表明，1-5 mM和20 mM醋酸钠显著抑制骨沉积，而10 mM则无此作用，这可能导致接受肾透析或全胃肠外营养的患者发生医源性代谢性骨病。
