1α,25-二羟基维生素D3（一种肾25-羟维生素D3 1α-羟化酶活性抑制剂）对大鼠肾脏和肝脏中CYP27 mRNA表达的影响。

Effects on CYP27 mRNA expression in rat kidney and liver by 1 alpha, 25-dihydroxyvitamin D3, a suppressor of renal 25-hydroxyvitamin D3 1 alpha-hydroxylase activity.

作者信息

Axén E, Postlind H, Wikvall K

机构信息

Department of Pharmaceutical Biosciences, University of Uppsala, Sweden.

出版信息

Biochem Biophys Res Commun. 1995 Oct 4;215(1):136-41. doi: 10.1006/bbrc.1995.2443.

DOI:10.1006/bbrc.1995.2443

PMID:7575580

Abstract

The production of 1 alpha,25-dihydroxyvitamin D3 is known to be down regulated by 1 alpha,25-dihydroxyvitamin D3 itself. It was recently shown that liver mitochondrial sterol 27-hydroxylase CYP27, present also in kidney, catalyzes 1 alpha-hydroxylation of 25-hydroxyvitamin D3. Treatment of vitamin D-deficient rats with a single i.v. dose of 1 alpha,25-dihydroxyvitamin D3 resulted in a marked suppression of CYP27 mRNA in kidney. The effects were not as pronounced as for CYP24. Liver CYP27 was not affected to the same extent. The results of the present communication indicate a coordinate regulation of CYP27 mRNA levels and 25-hydroxyvitamin D3 1 alpha-hydroxylase activity by 1 alpha,25-dihydroxyvitamin D3 in rat kidney.

摘要

已知1α,25 - 二羟基维生素D3的生成会受到其自身的下调作用。最近有研究表明，肝脏线粒体固醇27 - 羟化酶CYP27（肾脏中也存在）催化25 - 羟基维生素D3的1α - 羟化反应。用单次静脉注射剂量的1α,25 - 二羟基维生素D3处理维生素D缺乏的大鼠，会导致肾脏中CYP27 mRNA显著受抑制。其效果不如对CYP24的抑制那么明显。肝脏中的CYP27受影响程度不同。本研究结果表明，大鼠肾脏中CYP27 mRNA水平和25 - 羟基维生素D3 1α - 羟化酶活性受1α,25 - 二羟基维生素D3的协同调节。