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蚁蛉幼虫麻痹性多肽的纯化与特性分析

Purification and characterization of a paralytic polypeptide from larvae of Myrmeleon bore.

作者信息

Matsuda K, Suzuki H, Nakanishi F, Shio K, Komai K, Nishimura K

机构信息

Department of Agricultural Chemistry, Kinki University, Nara, Japan.

出版信息

Biochem Biophys Res Commun. 1995 Oct 4;215(1):167-71. doi: 10.1006/bbrc.1995.2448.

DOI:10.1006/bbrc.1995.2448
PMID:7575586
Abstract

A toxic substance was purified from larvae of the antilion, Myrmeleon bore, by DEAE Sephacel and Phenyl Superose column chromatography. The substance was a large polypeptide with a molecular weight of about 165-167 kDa. Its paralytic activity measured by injection against German cockroaches was about 130 times higher than that of tetrodotoxin on a molar basis.

摘要

通过DEAE Sephacel和苯基琼脂糖凝胶柱色谱法从蚁狮幼虫(Myrmeleon bore)中纯化出一种有毒物质。该物质是一种大分子多肽,分子量约为165 - 167 kDa。通过注射针对德国小蠊测定其麻痹活性,在摩尔基础上,其活性比河豚毒素高约130倍。

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