Siddiqi I, Funatomi H, Kobrin M S, Friess H, Büchler M W, Korc M
Department of Medicine, University of California, Irvine 92717, USA.
Biochem Biophys Res Commun. 1995 Oct 4;215(1):309-15. doi: 10.1006/bbrc.1995.2467.
Keratinocyte growth factor (KGF) is a member of the fibroblast growth factor (FGF) group of heparin-binding polypeptides. In the present study we sought to determine whether KGF is expressed in human pancreatic cancers. Using reverse transcriptase polymerase chain reaction (RT-PCR), a cDNA fragment of KGF was cloned and used to analyze Northern blots of RNA isolated from normal and cancerous human pancreatic tissues. Seven of 16 (44%) pancreatic cancer samples revealed significant overexpression of the 2.4 kilobase KGF mRNA transcript by comparison with the normal pancreas. Northern blot analysis failed to reveal the KGF transcript in several cultured human pancreatic cancer cell lines. However, by PCR analysis, some of the cell lines expressed KGF mRNA. Furthermore, 5 of 7 tested cell lines expressed the KGF receptor, and the growth of one cell line was enhanced by human recombinant KGF. These results suggest that KGF may participate in aberrant paracrine and autocrine pathways in human pancreatic cancer.
角质形成细胞生长因子（KGF）是肝素结合多肽的成纤维细胞生长因子（FGF）家族的一员。在本研究中，我们试图确定KGF是否在人类胰腺癌中表达。使用逆转录聚合酶链反应（RT-PCR），克隆了KGF的一个cDNA片段，并用于分析从正常和癌性人类胰腺组织中分离的RNA的Northern印迹。与正常胰腺相比，16个胰腺癌样本中的7个（44%）显示2.4千碱基KGF mRNA转录本有明显的过表达。Northern印迹分析未能在几种培养的人类胰腺癌细胞系中检测到KGF转录本。然而，通过PCR分析，一些细胞系表达KGF mRNA。此外，7个测试细胞系中的5个表达KGF受体，并且一种细胞系的生长被人重组KGF增强。这些结果表明KGF可能参与了人类胰腺癌中异常的旁分泌和自分泌途径。
