Pachman L M, Litt D L, Rowley A H, Hayford J R, Caliendo J, Heller S, Ticho B S, Christensen M, Patterson B, Ytterberg S R
Northwestern University Medical School, Chicago, Illinois, USA.
Arthritis Rheum. 1995 Oct;38(10):1513-8. doi: 10.1002/art.1780381019.
To investigate for the presence of increased titers of circulating antibody to putative infectious agents and for detectable viral RNA or bacterial DNA in children with active recent-onset juvenile dermatomyositis (DM).
Magnetic resonance imaging-directed muscle biopsies were performed in 20 children with active, untreated, recent-onset juvenile DM and in age-matched children with neurologic disease. Sera were tested for complement-fixing antibody to Coxsackievirus B (CVB), influenza A and B, parainfluenza 1 and 3, Mycoplasma pneumoniae, mumps, respiratory syncytial virus, and Reovirus; and by immunofluorescence for IgG antibody to Toxoplasma gondii cytomegalovirus and IgM antibody to Epstein-Barr virus. Muscle from juvenile DM patients and control children, CD-1 Swiss mice with and without CVB1 infection, and viral stock positive for CVB1-6 were tested using reverse-transcriptase polymerase chain reaction with 5 primer sets, 4 probes (1 Coxsackievirus, 3 Enterovirus), and universal primers for DNA.
No increased antibody, viral RNA, or bacterial DNA was present in the juvenile DM patients or the control children.
Juvenile DM may be triggered by unidentified agent(s) in the genetically susceptible host.
调查近期发病的活动性幼年皮肌炎（DM）患儿中，针对假定感染因子的循环抗体滴度升高情况以及是否可检测到病毒RNA或细菌DNA。
对20例近期发病、未经治疗的活动性幼年DM患儿以及年龄匹配的神经系统疾病患儿进行磁共振成像引导下的肌肉活检。检测血清中针对柯萨奇病毒B（CVB）、甲型和乙型流感病毒、副流感病毒1型和3型、肺炎支原体、腮腺炎病毒、呼吸道合胞病毒和呼肠孤病毒的补体结合抗体；通过免疫荧光检测弓形虫巨细胞病毒IgG抗体和爱泼斯坦-巴尔病毒IgM抗体。使用5组引物、4种探针（1种柯萨奇病毒、3种肠道病毒）以及DNA通用引物，通过逆转录聚合酶链反应检测幼年DM患者和对照儿童的肌肉、有无CVB1感染的CD-1瑞士小鼠以及CVB1-6病毒阳性储备液。
幼年DM患者和对照儿童中均未出现抗体、病毒RNA或细菌DNA升高的情况。
幼年DM可能由遗传易感宿主中不明因子引发。
