Marastoni M, Scaranari V, Fantini F, Sevignani C, Tomatis R
Dipartimento di Scienze Farmaceutiche dell' Università di Ferrara, Italy.
Arzneimittelforschung. 1995 Aug;45(8):891-3.
[D-Ala1]peptideT-amide, the linear hexapeptide H-Thr-Hse-Asn-Tyr-Thr-Asp-OH (LPT) and its cyclic analog, cyclo(-Thr-Hse-Asn-Tyr-Thr-Asp-) (CPT), were tested for their effects on the proliferation of cultured normal human keratinocytes (KTs) in comparison with vasoactive intestinal peptide (VIP). [D-Ala1]PT-NH2, LPT and VIP (all 0.1 mumol/l) increased the cell number in KT cultures, whereas CPT was ineffective. The VIP antagonist [N-Ac-Tyr1,D-Phe2]GRF (1-29)-NH2 significantly inhibited the VIP effects on KTs. On the other hand this antagonist did not affect the peptide T (PT) compounds-induced stimulation of KTs, providing indirect evidence that the mitogenic effects of VIP and PT peptides are probably mediated via different receptors.
将[D-Ala1]肽T-酰胺、线性六肽H-Thr-Hse-Asn-Tyr-Thr-Asp-OH（LPT）及其环类似物环（-Thr-Hse-Asn-Tyr-Thr-Asp-）（CPT）与血管活性肠肽（VIP）相比较，测试了它们对培养的正常人角质形成细胞（KTs）增殖的影响。[D-Ala1]PT-NH2、LPT和VIP（均为0.1μmol/L）增加了KT培养物中的细胞数量，而CPT则无效。VIP拮抗剂[N-Ac-Tyr1，D-Phe2]GRF（1-29）-NH2显著抑制了VIP对KTs的作用。另一方面，该拮抗剂不影响肽T（PT）化合物诱导的KTs刺激，间接证明VIP和PT肽的促有丝分裂作用可能通过不同的受体介导。
