Lah T T, Calaf G, Kalman E, Shinde B G, Russo J, Jarosz D, Zabrecky J, Somers R, Daskal I
Department of Pathology and Laboratory Medicine, Albert Einstein Medical Center, Philadelphia, Pennsylvania 19141, USA.
Biol Chem Hoppe Seyler. 1995 Jun;376(6):357-63. doi: 10.1515/bchm3.1995.376.6.357.
An increased expression of lysosomal enzymes, cathepsin (Cat) D, Cat B and Cat L, was observed in various human tumours and after in vitro cell transformation. To establish possible co-ordination in their expression, all three cathepsins were determined in human breast tumours and in transformed human breast epithelial cells (HBEC). In breast carcinoma (n = 120) all three cathepsins, determined immunochemically and by enzymatic activity, were increased compared to normal breast tissues. The activities, correlated with the corresponding protein masses for Cat D (r = 0.77, p < 0.01), but not for Cat B and Cat L. Significant increase in Cat B activity was observed in stage II compared to stage I tumours, and Cat L activity in stage III compared to stage II tumours, but no significant correlation of cathepsin protein with tumour stage (TNM) was established. No significant correlation between Cat D and the cysteine cathepsins B and L was observed. Similarly, Cat D, Cat B and Cat L levels did not correlate in the in vitro system, e.g. in the five transformed HBEC, such as evolved after dimethylbenz(a)anthracene treatment and c-Has-ras oncogene transfection of diploid MCF-10F cell line (Calaf et al., 1993). Transformed cells showed increased anchorage-independent growth and invasive capability (MCF-10 < MCF-10FTras < D3 < D3-1 < D3-1Tras). The intracellular level of Cat D was not related to cell invasiveness, while total cellular Cat B and Cat L increased 13 fold and 4 fold, respectively, in the most invasive cell line, D3-1Tras compared to MCF-10F.(ABSTRACT TRUNCATED AT 250 WORDS)
在各种人类肿瘤以及体外细胞转化后，观察到溶酶体酶组织蛋白酶（Cat）D、Cat B和Cat L的表达增加。为确定它们表达中可能存在的协同作用，在人类乳腺肿瘤和转化的人乳腺上皮细胞（HBEC）中对这三种组织蛋白酶进行了测定。与正常乳腺组织相比，在乳腺癌（n = 120）中，通过免疫化学和酶活性测定的所有三种组织蛋白酶均增加。Cat D的活性与相应蛋白质质量相关（r = 0.77，p < 0.01），但Cat B和Cat L并非如此。与I期肿瘤相比，II期肿瘤中Cat B活性显著增加；与II期肿瘤相比，III期肿瘤中Cat L活性显著增加，但未确定组织蛋白酶蛋白与肿瘤分期（TNM）之间存在显著相关性。未观察到Cat D与半胱氨酸组织蛋白酶B和L之间存在显著相关性。同样，在体外系统中，例如在五个转化的HBEC中，如经二甲基苯并（a）蒽处理和二倍体MCF - 10F细胞系的c - Has - ras癌基因转染后形成的细胞系中，Cat D、Cat B和Cat L水平也不相关（卡拉夫等人，1993年）。转化细胞显示出非锚定依赖性生长和侵袭能力增加（MCF - 10 < MCF - 10FTras < D3 < D3 - 1 < D3 - 1Tras）。Cat D的细胞内水平与细胞侵袭性无关，而在侵袭性最强的细胞系D3 - 1Tras中，与MCF - 10F相比，细胞总Cat B和Cat L分别增加了13倍和4倍。（摘要截断于250字）
