Kim Y, Adham I M, Haack T, Kremling H, Engel W
Institut für Humangenetik der Universität, Göttingen, Germany.
Biol Chem Hoppe Seyler. 1995 Jul;376(7):431-5. doi: 10.1515/bchm3.1995.376.7.431.
Outer dense fibers (ODF) or accessory fibers are filamentous structures of the sperm tail of many eumetozoan organisms endowed with internal fecundation. The bovine and porcine cDNA of an outer dense fiber protein was cloned, sequenced and compared to the previously characterized human and rat cDNA sequences. The coding sequences and the 5' and 3' untranslated regions of the ODF cDNAs are highly conserved. A comparison of the bovine, porcine, human and rat ODF protein sequences revealed that the protein displays a high degree of similarity, ranging from 87% to 98%. The ODF protein is rich in cysteine and contains the C.X.P. repeat at the C-terminal which is different in number among mammalian species. All the 27 cysteine residues in the ODF sequence except those in the C.X.P. repeat are conserved in the four species. We report here also the organization of the bovine ODF gene which is similar to that of human and rat. The transcription start site in the bovine ODF gene is localized 98 bp upstream of the translation start site. Alignment of the 5' flanking region of bovine ODF with the rat gene reveals that the first 130 nucleotides upstream of the transcription start site exhibit an overall sequence similarity of 83%. This conserved region contains a TATA-like box (TTTAAA) and binding sites for AFT/CREB and EGR-1 transcription factors.
外致密纤维（ODF）或附属纤维是许多具有体内受精能力的真后生动物精子尾部的丝状结构。克隆并测序了一种外致密纤维蛋白的牛和猪cDNA，并将其与先前鉴定的人和大鼠cDNA序列进行比较。ODF cDNA的编码序列以及5'和3'非翻译区高度保守。对牛、猪、人及大鼠的ODF蛋白序列进行比较后发现，该蛋白具有高度相似性，相似度在87%至98%之间。ODF蛋白富含半胱氨酸，且在C端含有C.X.P.重复序列，该重复序列在哺乳动物物种中的数量有所不同。ODF序列中的所有27个半胱氨酸残基（C.X.P.重复序列中的除外）在这四个物种中都是保守的。我们在此还报告了牛ODF基因的结构，它与人和大鼠的相似。牛ODF基因的转录起始位点位于翻译起始位点上游98 bp处。牛ODF的5'侧翼区与大鼠基因的比对显示，转录起始位点上游的前130个核苷酸总体序列相似度为83%。该保守区域包含一个类TATA盒（TTTAAA）以及AFT/CREB和EGR-1转录因子的结合位点。
