Petersen C, Füzesi L, Hoyer-Fender S
III Zoologisches Institut-Entwicklungsbiologie, Universität Göttingen, Humboldtallee 34A, 37073 Göttingen, Germany.
Mol Hum Reprod. 1999 Jul;5(7):627-35. doi: 10.1093/molehr/5.7.627.
The outer dense fibres (ODF) are a main cytoskeletal structure of the sperm tail. Despite their importance in the morphology and function of the sperm tail, their constituents are poorly described. Here we investigate the protein composition of human outer dense fibres. Our results suggest that human ODF consist of about 10 major and of at least 15 minor proteins, where all major proteins are ODF1, ODF2 or ODF2-related proteins. From a human testis cDNA library, we isolated two slightly different cDNAs encoding ODF2 proteins of approximately 70 kDa. Human ODF2 cDNAs and their encoded proteins are very similar to those isolated from rat and mouse pointing to a high evolutionary pressure residing on these proteins. Transcription of ODF2 is restricted to testis tissue and more specifically to round spermatids as was demonstrated by a non-radioactive in-situ hybridization. ODF2 proteins were detected in the sperm tail. Their distribution along the length of the sperm tail shows that the ODF normally extend to about half the principal piece of the sperm tail. The former result opens the possibility for a screening regarding the distribution of sperm tail proteins related to motility disorders.
外周致密纤维（ODF）是精子尾部的主要细胞骨架结构。尽管它们在精子尾部的形态和功能中很重要，但其组成成分却鲜有描述。在此，我们研究了人类外周致密纤维的蛋白质组成。我们的结果表明，人类ODF由约10种主要蛋白质和至少15种次要蛋白质组成，其中所有主要蛋白质均为ODF1、ODF2或ODF2相关蛋白质。从人类睾丸cDNA文库中，我们分离出两个略有不同的cDNA，它们编码约70 kDa的ODF2蛋白质。人类ODF2 cDNA及其编码的蛋白质与从大鼠和小鼠中分离出的非常相似，这表明这些蛋白质存在高度的进化压力。如通过非放射性原位杂交所证明的，ODF2的转录仅限于睾丸组织，更具体地说是圆形精子细胞。在精子尾部检测到了ODF2蛋白质。它们沿精子尾部长度的分布表明，ODF通常延伸至精子尾部主段的大约一半长度处。前一个结果为筛查与运动障碍相关的精子尾部蛋白质的分布提供了可能性。
