Zon G
Lynx Therapeutics, Inc., Hayward, CA 94545, USA.
Mol Neurobiol. 1995 Apr-Jun;10(2-3):219-29. doi: 10.1007/BF02740677.
Over the past several years, the use of synthetic oligonucleotides and functional analogs thereof as a possibly general means of controlling genetic expression has received widespread attention. Following a brief overview of some of the basic principles and strategies for this approach, attention is focused here on summarizing some recent reports of in vitro and, in particular, in vivo investigations in various animal models using phosphorothioate analogs of 2'-deoxyoligonucleotides. In view of these findings, which include studies related to neurobiology, this field should find significant utility in applications of the antisense method for controlling genetic expression.
在过去几年中，合成寡核苷酸及其功能类似物作为一种可能通用的控制基因表达的手段受到了广泛关注。在简要概述了这种方法的一些基本原理和策略之后，本文重点总结了一些最近关于使用2'-脱氧寡核苷酸的硫代磷酸酯类似物在各种动物模型中进行体外研究，特别是体内研究的报告。鉴于这些包括与神经生物学相关研究在内的发现，该领域在反义方法控制基因表达的应用中应具有显著的实用性。
