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从炼油厂附近土壤中分离和鉴定四种多环芳烃降解细菌。

Isolation and characterization of four polycyclic aromatic hydrocarbon degrading bacteria from soil near an oil refinery.

作者信息

Ashok B T, Saxena S, Musarrat J

机构信息

Interdisciplinary Biotechnology Unit, AMU, Aligarh, Uttar Pradesh, India.

出版信息

Lett Appl Microbiol. 1995 Oct;21(4):246-8. doi: 10.1111/j.1472-765x.1995.tb01052.x.

DOI:10.1111/j.1472-765x.1995.tb01052.x
PMID:7576515
Abstract

Four bacterial strains (I-IV) capable of optimum growth on 0.1% naphthalene, anthracene or a mixture of naphthalene and phenanthrene were isolated from soil near an oil refinery. Two isolates (I and II) were identified as belonging to the genus Micrococcus, while strains III and IV were identified as Pseudomonas and Alcaligenes respectively. All the isolates were found to bear high molecular weight plasmid DNA (isolate I and IV 89%, II 67.5% and III 92.1% of lambda DNA), which is presumed to aid in the metabolism of polycyclic aromatic hydrocarbons. The strains also showed appreciable growth at high concentrations of NaCl (up to 7.5%).

摘要

从炼油厂附近的土壤中分离出了四种能够在0.1%萘、蒽或萘与菲的混合物上实现最佳生长的细菌菌株(I-IV)。其中两个分离株(I和II)被鉴定为属于微球菌属,而菌株III和IV分别被鉴定为假单胞菌属和产碱菌属。所有分离株均被发现携带高分子量质粒DNA(分离株I和IV为λDNA的89%,II为67.5%,III为92.1%),推测这有助于多环芳烃的代谢。这些菌株在高浓度NaCl(高达7.5%)下也表现出明显的生长。

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