Trzesicka-Mlynarz D, Ward O P
Department of Biology, University of Waterloo, ON, Canada.
Can J Microbiol. 1995 Jun;41(6):470-6. doi: 10.1139/m95-063.
A mixed culture, isolated from soil contaminated with polycyclic aromatic hydrocarbons (PAHs), grew on and degraded fluoranthene in aqueous media supplemented with glucose, yeast extract, and peptone. Increased complex nitrogen levels in the medium promoted bacterial growth and a greater extent of fluoranthene degradation. Amendment of the media with high glucose levels also diminished specific fluoranthene degradation. The mixed culture was capable of degrading a range of other PAHs, including benzo[a]pyrene, anthracene, phenanthrene, acenaphthene, and fluorene. The mixed culture contained four predominant isolates, all of which were Gram-negative rods, three of which were identified as Pseudomonas putida, Flavobacterium sp., and Pseudomonas aeruginosa. Better degradation of a defined PAH mixture was observed with the mixed culture than with individual isolates. A reconstituted culture, prepared by combining the four individual isolates, manifested a similar PAH biodegradation performance to the original mixed culture. When compared with the mixed culture, individual isolates exhibited a relatively good capacity to remove more water-soluble PAHs (acenaphthene, fluorene, phenanthrene, fluoranthene). In contrast, removal of less water-soluble PAHs (anthracene and pyrene) was low or negligible with isolated cultures compared with the mixed culture.
从受多环芳烃（PAHs）污染的土壤中分离出的一种混合培养物，在添加了葡萄糖、酵母提取物和蛋白胨的水介质中能够生长并降解荧蒽。培养基中复合氮水平的提高促进了细菌生长以及荧蒽的更大程度降解。高葡萄糖水平对培养基的改良也降低了荧蒽的特定降解率。该混合培养物能够降解一系列其他PAHs，包括苯并[a]芘、蒽、菲、苊和芴。该混合培养物包含四种主要分离菌株，均为革兰氏阴性杆菌，其中三种被鉴定为恶臭假单胞菌、黄杆菌属和铜绿假单胞菌。与单个分离菌株相比，混合培养物对特定PAH混合物的降解效果更好。通过将四种单个分离菌株组合制备的重组培养物表现出与原始混合培养物相似的PAH生物降解性能。与混合培养物相比，单个分离菌株表现出相对较好的去除更多水溶性PAHs（苊、芴、菲、荧蒽）的能力。相比之下，与混合培养物相比，分离培养物对低水溶性PAHs（蒽和芘）的去除率较低或可忽略不计。
