Thomas P G, Ignotz G G, Ball B A, Miller P G, Brinsko S P, Currie B
Department of Clinical Sciences, Cornell University, Ithaca, New York, USA.
Mol Reprod Dev. 1995 Aug;41(4):468-78. doi: 10.1002/mrd.1080410409.
Oviduct epithelial cells (OEC) increasingly are used to support embryonic development and to study gamete interactions with the female reproductive tract in vitro. This series of experiments was designed to characterize monolayers derived from oviduct epithelium. Epithelial cells harvested from the isthmus and ampulla of the oviducts of five estrous mares were cultured with or without the basal lamina extract, Matrigel. Within each group OEC were cultured in the presence of either estradiol-17 beta or a carrier control. All groups were subcultured three times. Epithelial cell morphology and function were examined by microscopy, analysis of secreted proteins, and immunocytochemistry. Epithelial cells attached more rapidly and reached confluence sooner when cultured on Matrigel than in uncoated wells. Cells showed variable evidence of ciliary activity up to 12 days in primary culture. Cells grown on Matrigel had a more polarized appearance in primary culture than those in uncoated wells, although no morphologic difference between anatomic site of origin or between steroid treated groups was noted. Anatomic site of origin had no effect, and steroid treatment had minimal effects, on patterns of secreted proteins. However, some differences were noted in protein secretion between cells grown with or without Matrigel. These data suggest that culture substrate may affect structure and function of OEC monolayers.
输卵管上皮细胞（OEC）越来越多地被用于支持胚胎发育，并在体外研究配子与雌性生殖道的相互作用。这一系列实验旨在对源自输卵管上皮的单层细胞进行特性描述。从五匹处于发情期母马的输卵管峡部和壶腹部采集的上皮细胞，在有或没有基膜提取物基质胶的情况下进行培养。在每组中，OEC在17β-雌二醇或载体对照存在的情况下进行培养。所有组均传代培养三次。通过显微镜检查、分泌蛋白分析和免疫细胞化学来检测上皮细胞的形态和功能。与在未包被的孔中培养相比，在基质胶上培养时上皮细胞附着更快且更早达到汇合。在原代培养中，细胞在长达12天的时间内显示出不同程度的纤毛活动迹象。在原代培养中，在基质胶上生长的细胞比在未包被孔中的细胞具有更极化的外观，尽管未观察到起源解剖部位或类固醇处理组之间的形态学差异。起源解剖部位对分泌蛋白模式没有影响，类固醇处理的影响也最小。然而，在有或没有基质胶培养的细胞之间，在蛋白质分泌方面存在一些差异。这些数据表明培养底物可能会影响OEC单层细胞的结构和功能。
