Bolwig G M, Del Vecchio M, Hannon G, Tully T
Cold Spring Harbor Laboratory, New York 11724, USA.
Neuron. 1995 Oct;15(4):829-42. doi: 10.1016/0896-6273(95)90174-4.
The linotte (lio) gene was identified in a screen for mutations that disrupted 3 hr memory after olfactory associative learning, without affecting the perception of odors or electroshock. The mutagenesis yielded a transposon-tagged gene disruption, which allowed rapid cloning of genomic DNA. The lio transcription unit was identified via rescue of the lio1 learning/memory defect by induced expression of a lio+ transgene in adults. The perception of odors or electroshock remained normal when the lio+ transgene was expressed in these lio1 flies. Learning/memory remained normal when the lio+ transgene was expressed in wild-type (lio+) flies. The lio gene produces only one transcript, the level of expression of which varies throughout development. Sequence analysis indicates that lio encodes a novel protein.
在一项筛选中鉴定出了雀鹀（lio）基因，该筛选针对的是在嗅觉联想学习后破坏3小时记忆但不影响气味感知或电击的突变。诱变产生了一个转座子标记的基因破坏，这使得能够快速克隆基因组DNA。通过在成虫中诱导表达lio +转基因来挽救lio1学习/记忆缺陷，从而鉴定出lio转录单位。当在这些lio1果蝇中表达lio +转基因时，气味或电击的感知仍保持正常。当在野生型（lio +）果蝇中表达lio +转基因时，学习/记忆仍保持正常。lio基因仅产生一种转录本，其表达水平在整个发育过程中有所不同。序列分析表明lio编码一种新型蛋白质。
