Wilkinson S R, Young D I, Morris J G, Young M
Institute of Biological Sciences, University of Wales, Aberystwyth, UK.
FEMS Microbiol Rev. 1995 Oct;17(3):275-85. doi: 10.1111/j.1574-6976.1995.tb00211.x.
A physical map of the Clostridium beijerinckii (formerly Clostridium acetobutylicum) NCIMB 8052 chromosome has been constructed, encompassing about 90 rare restriction sites. The 14 rrn operons together with about 40 genes have been assigned positions on the map. Genetic analysis and gene transfer have been developed in this organism to enable in vivo analysis of the roles of cloned genes using marker replacement technology. Experiments using the available genetic tools have shown that spo0A plays a cardinal role in controlling several aspects of the transition from exponential growth to stationary phase in C. beijerinckii. These include initiation of sporulation, accumulation of the storage polysaccharide, granulose, and production of acetone and butanol. Several C. beijerinckii and C. acetobutylicum genes concerned with fermentative metabolism, whose expression is modulated at the onset of solventogenesis, contain sequence motifs resembling 0A boxes in their 5' regulatory regions. This invites the speculation that they are under the direct control of Spo0A, and additional data are now required to test this prediction.
已构建了拜氏梭菌（以前称为丙酮丁醇梭菌）NCIMB 8052染色体的物理图谱，该图谱包含约90个稀有限制性酶切位点。14个rrn操纵子以及约40个基因已在图谱上定位。已在该生物体中开发了遗传分析和基因转移技术，以利用标记替换技术对克隆基因的作用进行体内分析。使用现有遗传工具进行的实验表明，spo0A在控制拜氏梭菌从指数生长期到稳定期转变的多个方面起着关键作用。这些方面包括芽孢形成的起始、储存多糖颗粒淀粉的积累以及丙酮和丁醇的产生。几个与发酵代谢有关的拜氏梭菌和丙酮丁醇梭菌基因，其表达在溶剂生成开始时受到调节，在其5'调控区域含有类似于0A框的序列基序。这引发了一种推测，即它们受Spo0A的直接控制，现在需要更多数据来验证这一预测。
