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拜氏梭菌（原丙酮丁醇梭菌）NCIMB 8052染色体的物理图谱。

Physical map of the Clostridium beijerinckii (formerly Clostridium acetobutylicum) NCIMB 8052 chromosome.

作者信息

Wilkinson S R, Young M

机构信息

Institute of Biological Sciences, University of Wales, Aberystwyth, Dyfed, United Kingdom.

出版信息

J Bacteriol. 1995 Jan;177(2):439-48. doi: 10.1128/jb.177.2.439-448.1995.

DOI:10.1128/jb.177.2.439-448.1995
PMID:7814334
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC176608/
Abstract

A combined physical and genetic map of the single, circular, 6.7-Mbp chromosome of the NCIMB 8052 strain of Clostridium beijerinckii (formerly Clostridium acetobutylicum) has been constructed by using a combination of cloned DNA fragments as hybridization probes and a bank of strains harboring insertions of the conjugative transposon Tn1545. The positions of 81 restriction endonuclease cleavage sites and 32 genes have been determined. Eight genes concerned with solventogenic fermentation are found at three different locations. The chromosome contains at least 13 rrn operons, 11 of which have been located on the map. Their transcriptional orientation diverges from the presumed location of the replication origin.

摘要

利用克隆的DNA片段作为杂交探针，并结合携带接合转座子Tn1545插入片段的菌株库，构建了拜氏梭菌（以前称为丙酮丁醇梭菌）NCIMB 8052菌株单一环状6.7-Mbp染色体的物理图谱和遗传图谱。已确定了81个限制性内切酶切割位点和32个基因的位置。在三个不同位置发现了8个与产溶剂发酵有关的基因。该染色体至少包含13个rrn操纵子，其中11个已定位在图谱上。它们的转录方向与推测的复制起点位置不同。