Schmid M F, Jakana J, Chiu W, Matsudaira P
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA.
J Struct Biol. 1995 Sep-Oct;115(2):209-13. doi: 10.1006/jsbi.1995.1045.
The acrosomal bundle from Limulus sperm has been imaged to 7 A in a 400-kV electron cryomicroscope using the spot-scan technique. These images have been processed by Fourier averaging, corrected for the contrast transfer function, and merged to that resolution. The reconstructed density map of this projection shows features consistent with the 13-A projection map from a helical reconstruction and with the X-ray-derived model for F-actin, and regions of possible scruin-scruin contacts.
使用斑点扫描技术，在400 kV电子冷冻显微镜中对鲎精子的顶体束进行成像，分辨率达到7埃。这些图像经过傅里叶平均处理，校正了对比度传递函数，并合并到该分辨率。此投影的重建密度图显示的特征与螺旋重建得到的13埃投影图以及F-肌动蛋白的X射线衍生模型一致，还有可能存在的scruin-scruin接触区域。
