使用400 kV电子冷冻显微镜以7 Å分辨率对鲎精子的冷冻水合顶体束进行成像。
Imaging frozen, hydrated acrosomal bundle from Limulus sperm at 7 A resolution with a 400 kV electron cryomicroscope.
作者信息
Schmid M F, Jakana J, Matsudaira P, Chiu W
机构信息
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
出版信息
J Mol Biol. 1993 Mar 20;230(2):384-6. doi: 10.1006/jmbi.1993.1155.
PMID:8464053
Abstract
Actin and scruin are present in a highly ordered array in the acrosomal bundle from Limulus sperm. Frozen, hydrated acrosomal bundle can be imaged in a 400 kV electron cryomicroscope using the spot-scan technique. Quantitative analysis of the h0l projection images shows diffraction data beyond 7 A.
摘要
在鲎精子的顶体束中，肌动蛋白和丝团蛋白以高度有序的阵列形式存在。冷冻、水合的顶体束可以使用点扫描技术在400 kV电子冷冻显微镜下成像。对h0l投影图像的定量分析显示了超过7埃的衍射数据。
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