Gianguzza F, Casano C, Ragusa M
Dipartimento di Biologia Cellulare e dello Sviluppo, Palermo, Italy.
Int J Dev Biol. 1995 Jun;39(3):477-83.
We have used Northern blot and whole-mount in situ hybridizations to analyze the temporal and spatial expression pattern of the Pl alpha 2 alpha-tubulin gene in Paracentrotus lividus sea urchin embryos. The Pl alpha 2 transcript is first detectable at 14 h post-fertilization (blastula stage) and it is only expressed in the oral ectoderm. The amount of transcripts of this gene increases throughout development and accumulates up to the pluteus stage. In this stage the Pl alpha 2 transcript is localized in the neural structures of the embryo. We conclude that the Pl alpha 2 gene is an early neurogenic territory marker. Furthermore we have observed the same localization of the Pl alpha 2 transcript in the Zn(++)- or phenytoin-treated embryos, confirming the animal localization of the Pl alpha 2 transcript and its specific relation to neurogenic territory, whose differentiation starts from few founder cells present at blastula stage.
我们运用Northern印迹法和整体原位杂交技术，分析了紫球海胆胚胎中Plα2α-微管蛋白基因的时空表达模式。Plα2转录本在受精后14小时（囊胚期）首次可检测到，且仅在口外胚层中表达。该基因的转录本数量在整个发育过程中增加，并积累至长腕幼虫期。在此阶段，Plα2转录本定位于胚胎的神经结构中。我们得出结论，Plα2基因是早期神经发生区域的标志物。此外，我们在经锌离子或苯妥英处理的胚胎中观察到了Plα2转录本的相同定位，证实了Plα2转录本的动物性定位及其与神经发生区域的特定关系，其分化始于囊胚期存在的少数奠基细胞。
