Biesterfeld S, Pennings K, Grussendorf-Conen E I, Böcking A
Institute of Pathology, Technical University of Aachen, Germany.
Br J Dermatol. 1995 Oct;133(4):557-60. doi: 10.1111/j.1365-2133.1995.tb02704.x.
The value of DNA single cell cytometry for the detection of aneuploidy was assessed in 100 specimens of actinic keratoses and 39 specimens of Bowen's disease. Ten seborrhoeic keratoses and 10 samples of normal epidermis served as negative control groups. Monolayer smears, prepared from formalin-fixed, paraffin-embedded tissues, were Feulgen-stained and used for interactive DNA-cytometry. In each specimen, the DNA content of 150 randomly chosen squamous epithelial cells was measured, using a TV-image analysis system (TAS-plus, Leica, Germany). Aneuploidy was diagnosed if at least three nuclei with a DNA content above 5c (5cEE > or = 3) were found. The aneuploidy rate in actinic keratosis was 69% (69 of 100) and in Bowen's disease was 95% (37 of 39). Another 20 specimens of actinic keratoses and the remaining two specimens of Bowen's disease were diagnosed as suspicious for aneuploidy (0 < 5cEE < 3). The 20 specimens of seborrhoeic keratoses and normal epidermis did not show any nuclei above the 5c level, and were classified as non-aneuploid. This indicates a sensitivity of 76% (106 of 139) and a specificity of 100% (20 of 20). The frequent occurrence of aneuploidy in actinic keratoses and Bowen's disease underlines the character of the lesions as epidermal carcinomas in situ, but does not explain the long-term low incidence of invasive growth.
在100例光化性角化病标本和39例鲍温病标本中评估了DNA单细胞流式细胞术检测非整倍体的价值。10例脂溢性角化病和10例正常表皮样本作为阴性对照组。从福尔马林固定、石蜡包埋的组织制备单层涂片，进行Feulgen染色并用于交互式DNA流式细胞术。在每个标本中，使用电视图像分析系统（TAS-plus，德国徕卡）测量150个随机选择的鳞状上皮细胞的DNA含量。如果发现至少三个DNA含量高于5c的细胞核（5cEE≥3），则诊断为非整倍体。光化性角化病的非整倍体率为69%（100例中的69例），鲍温病为95%（39例中的37例）。另外20例光化性角化病标本和其余2例鲍温病标本被诊断为非整倍体可疑（0＜5cEE＜3）。20例脂溢性角化病和正常表皮标本未显示任何高于5c水平的细胞核，被归类为非非整倍体。这表明敏感性为76%（139例中的106例），特异性为100%（20例中的20例）。光化性角化病和鲍温病中非整倍体的频繁出现突出了这些病变作为原位表皮癌的特征，但无法解释侵袭性生长的长期低发生率。
