Motzny C K, Holmgren R
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208-3500, USA.
Mech Dev. 1995 Jul;52(1):137-50. doi: 10.1016/0925-4773(95)00397-j.
The segment polarity gene cubitus interruptus (ci) is required to maintain expression of the wingless gene and to specify naked cuticle within each epidermal segment. Antibodies were generated against the ci protein and used to analyze its pattern of expression. By stage 11, post-transcriptional regulation of ci is observed. ci transcript levels are uniform across the anterior compartment, but protein levels are higher next to the compartment boundaries. The distribution of the ci protein is altered in fused, hedgehog and wingless mutants suggesting cell-cell signaling may regulate ci protein levels. The role of ci in cell-cell signaling and pattern formation was examined in double mutants of ci with patched and zeste-white3/shaggy.
节段极性基因肘脉中断（ci）对于维持无翅基因的表达以及确定每个表皮节段内的裸表皮是必需的。制备了针对ci蛋白的抗体，并用于分析其表达模式。到第11阶段，观察到ci的转录后调控。ci转录本水平在前部区域是均匀的，但蛋白水平在区域边界附近更高。在融合、刺猬和无翅突变体中，ci蛋白的分布发生了改变，这表明细胞间信号传导可能调节ci蛋白水平。在ci与patched和zeste-white3/shaggy的双突变体中研究了ci在细胞间信号传导和模式形成中的作用。
