Distamycin对人雌激素受体基因表达的改变

Alteration of the expression of human estrogen receptor gene by distamycin.

作者信息

Bianchi N, Passadore M, Feriotto G, Mischiati C, Gambari R, Piva R

机构信息

Dipartimento di Biochimica e Biologia Molecolare, Università degli Studi di Ferrara, Italy.

出版信息

J Steroid Biochem Mol Biol. 1995 Sep;54(5-6):211-5. doi: 10.1016/0960-0760(95)00133-k.

DOI:10.1016/0960-0760(95)00133-k

PMID:7577702

Abstract

The effects of distamycin on the expression of the estrogen receptor gene were determined in the MCF7 human breast cancer cell line. Estrogen receptor (ER) RNA transcripts were analyzed by Northern blotting and RT-PCR using specific oligonucleotides for the 5' upstream region and for ER cDNA. After ex vivo distamycin treatment of the cells the expression of the canonical ER mRNA isoform of 6.3 kb is strongly inhibited, without appreciable alteration of the accumulation of 5' upstream ER mRNA isoforms. These results suggest that distamycin alters the transcriptional activity of the ER gene causing a change in the ratio between the canonical transcript and other isoforms containing 5' upstream regions.

摘要

在MCF7人乳腺癌细胞系中测定了偏端霉素对雌激素受体基因表达的影响。使用针对5'上游区域和雌激素受体（ER）cDNA的特异性寡核苷酸，通过Northern印迹法和逆转录-聚合酶链反应（RT-PCR）分析雌激素受体（ER）RNA转录本。在对细胞进行偏端霉素离体处理后，6.3 kb的经典ER mRNA亚型的表达受到强烈抑制，而5'上游ER mRNA亚型的积累没有明显改变。这些结果表明，偏端霉素改变了ER基因的转录活性，导致经典转录本与其他含有5'上游区域的亚型之间的比例发生变化。