Malkusch W, Konerding M A, Klapthor B, Bruch J
Institut für Hygiene und Arbeitsmedizin, Universitätsklinikum Essen, Germany.
Anal Cell Pathol. 1995 Jul;9(1):69-81.
Microvascular corrosion casting is an established method to investigate vascular patterns of nearly all organs. Trying to evaluate these specimens quantitatively using native scanning electron microscope (SEM) pictures always implied a big error, because the information of depth cannot be taken into account. However, SEM stereo pairs allow for exact measurements. Tumour microcorrosion casts were used to demonstrate the feasibility of this 3-D quantification method. The information of depth was calculated for each measuring point using the parallax. From the resulting coordinates the intervascular distances, the interbranching distances as well as the interbranching angles were determined. We found significant differences between all investigated tumours. Reproducibility tests and tests for the greatest error possibilities resulted in a maximum deviation of 2.5% to be expected. Consequently this method is suitable for all application ranges of microvascular corrosion casting as a quantitative determination method.
微血管铸型是一种用于研究几乎所有器官血管模式的既定方法。试图使用原生扫描电子显微镜（SEM）图像对这些标本进行定量评估总是存在很大误差，因为无法考虑深度信息。然而，SEM立体对允许进行精确测量。肿瘤微血管铸型被用于证明这种三维定量方法的可行性。利用视差为每个测量点计算深度信息。根据所得坐标确定血管间距离、分支间距离以及分支间角度。我们发现所有研究的肿瘤之间存在显著差异。重复性测试和最大误差可能性测试结果显示预期最大偏差为2.5%。因此，这种方法适用于微血管铸型作为定量测定方法的所有应用范围。
