Iwahara S, Satoh H, Song D X, Webb J, Burlingame A L, Nagae Y, Muller-Eberhard U
Department of Pediatrics, Cornell University Medical College, New York, New York 10021, USA.
Biochemistry. 1995 Oct 17;34(41):13398-406. doi: 10.1021/bi00041a017.
A heme-binding protein (designated HBP23) has been purified from rat liver cytosol using heme-affinity chromatography and either reverse-phase high-performance liquid chromatography or sequential ion-exchange chromatography. The protein (23 kDa) binds heme with an affinity (Kd = 55 nM) higher than that of the abundant cytosolic heme-binding proteins, heme-binding protein (HBP)/liver fatty acid-binding protein (L-FABP) and the glutathione S-transferases (GSTs) (Kd = 100-200 nM). HBP23 is present in the cytosol of liver, kidney, spleen, small intestine, and heart, with the liver showing the highest content. A cDNA coding the 23-kDa protein was cloned using reverse transcription polymerase chain reaction with degenerative oligonucleotides derived from partial amino acid sequences. The cloned cDNA encoded 199 amino acids, and its amino acid sequence showed no homology to HBP/L-FABP, GSTs, or any other heme-binding proteins or hemeproteins. Homology search showed that HBP23 is highly homologous to mouse macrophage 23-kDa stress protein, which is inducible by oxidant stress in peritoneal macrophages [Ishii, T., Yamada, M., Sato, H., Matsue, M., Taketani, S., Nakayama, K., Sugita, Y., and Bannai, S. (1993) J. Biol. Chem. 268, 18633-18636]. Thioredoxin peroxidase as well as HBP23 and the mouse macrophage 23-kDa stress protein are members of the peroxiredoxin family, a recently recognized class of antioxidant proteins [Chae, H. Z., Chung, S. J., & Rhee, S. G. (1994) J. Biol. Chem. 269, 27670-27678]. An increase in HBP23 mRNA was observed in Hepa 1-6 cells after treatment with heme and cadmium and during liver regeneration after partial hepatectomy.(ABSTRACT TRUNCATED AT 250 WORDS)
一种血红素结合蛋白（命名为HBP23）已通过血红素亲和层析以及反相高效液相色谱法或连续离子交换色谱法从大鼠肝脏胞质溶胶中纯化出来。该蛋白（23 kDa）与血红素结合的亲和力（Kd = 55 nM）高于丰富的胞质血红素结合蛋白、血红素结合蛋白（HBP）/肝脏脂肪酸结合蛋白（L-FABP）和谷胱甘肽S-转移酶（GSTs）（Kd = 100 - 200 nM）。HBP23存在于肝脏、肾脏、脾脏、小肠和心脏的胞质溶胶中，其中肝脏中的含量最高。利用逆转录聚合酶链反应，采用从部分氨基酸序列推导而来的简并寡核苷酸，克隆了编码23 kDa蛋白的cDNA。克隆的cDNA编码199个氨基酸，其氨基酸序列与HBP/L-FABP、GSTs或任何其他血红素结合蛋白或血红素蛋白均无同源性。同源性搜索表明，HBP23与小鼠巨噬细胞23 kDa应激蛋白高度同源，后者可由腹膜巨噬细胞中的氧化应激诱导产生[石井，T.，山田，M.，佐藤，H.，松江，M.，武谷，S.，中山，K.，杉田，Y.，及伴内，S.（1993）《生物化学杂志》268，18633 - 18636]。硫氧还蛋白过氧化物酶以及HBP23和小鼠巨噬细胞23 kDa应激蛋白都是过氧化物酶家族的成员，这是最近才被认识的一类抗氧化蛋白[蔡，H. Z.，郑，S. J.，及李，S. G.（1994）《生物化学杂志》269，27670 - 27678]。在用血红素和镉处理后以及部分肝切除术后肝脏再生过程中，Hepa 1 - 6细胞中观察到HBP23 mRNA增加。（摘要截断于250字）
