Sidorova N N, Usmanov R A, Kochetov G A
Biokhimiia. 1995 Jul;60(7):1095-9.
Modification of the method of Holzer et al. for the synthesis and purification of dihydroxyethylthiamine pyrophosphate, an intermediate of the transketolase reaction, is described. Absorption and circular dichroism spectra as well as the molar coefficient of circular dichroism absorption at 273 nm are presented. A convenient and rapid procedure for quantitative determination of dihydroxyethylthiamine pyrophosphate in solution where it is present as the only component or as a mixture with thiamine pyrophosphate (or other optically inactive compounds), is proposed.
本文描述了对霍尔泽等人合成和纯化转酮醇酶反应中间体二羟乙基硫胺焦磷酸方法的改进。给出了吸收光谱、圆二色光谱以及在273nm处的圆二色吸收摩尔系数。提出了一种简便快速的方法，用于定量测定溶液中作为唯一成分或与硫胺焦磷酸（或其他光学惰性化合物）混合存在的二羟乙基硫胺焦磷酸。
