Sidorova N N, Usmanov R A, Kochetov G A
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
Biochem Mol Biol Int. 1995 Jan;35(1):111-5.
This work is concerned with a convenient and fast method for quantitative determination of dihydroxyethylthiamine pyrophosphate in solution where it is present as the only component or as a mixture with thiamine pyrophosphate (or other optically inactive compounds). The molar coefficient is given for circular dichroism absorption of dihydroxyethylthiamine pyrophosphate. The method for determining thiamine pyrophosphate in the presence of dihydroxyethylthiamine pyrophosphate is described as well.
本研究涉及一种便捷快速的方法，用于定量测定溶液中的二羟乙基硫胺焦磷酸，该溶液中它作为唯一成分存在，或与硫胺焦磷酸（或其他非光学活性化合物）混合存在。给出了二羟乙基硫胺焦磷酸圆二色吸收的摩尔系数。还描述了在存在二羟乙基硫胺焦磷酸的情况下测定硫胺焦磷酸的方法。
