Lundquist P, Kågedal B, Nilsson L
Department of Clinical Chemistry, University Hospital, Linköping, Sweden.
Eur J Clin Chem Clin Biochem. 1995 Jun;33(6):343-9. doi: 10.1515/cclm.1995.33.6.343.
An improved spectrophotometric method is described for the determination of thiocyanate in plasma and urine. Thiocyanate is adsorbed on a weak anion-exchange resin with strong affinity for chaotropic ions, and eluted with perchlorate. Thiocyanate is then chlorinated by hypochlorite and quantified according to the König (J Prakt Chem 1904; 69:105-37) reaction by use of isonicotinic acid and 1,3-dimethyl-barbituric acid. The method affords a simple, rapid and sensitive assay for thiocyanate and has a detection limit of 0.93 mumol/l. At thiocyanate concentrations of 107.1 and 167.4 mumol/l in plasma and urine the within-day CVs were 0.69% and 1.1% respectively, and the total imprecision measured for a period of 65 days was 0.98%. Analytical recoveries were quantitative both with urine and plasma samples.
本文描述了一种改进的分光光度法，用于测定血浆和尿液中的硫氰酸盐。硫氰酸盐被一种对离液剂离子具有强亲和力的弱阴离子交换树脂吸附，并用高氯酸盐洗脱。然后，硫氰酸盐用次氯酸盐氯化，并根据König反应（《实用化学杂志》1904年；69:105 - 37），使用异烟酸和1,3 - 二甲基巴比妥酸进行定量。该方法为硫氰酸盐提供了一种简单、快速且灵敏的测定方法，检测限为0.93μmol/L。在血浆和尿液中硫氰酸盐浓度分别为107.1和167.4μmol/L时，日内变异系数分别为0.69%和1.1%，在65天内测得的总不精密度为0.98%。尿液和血浆样品的分析回收率均为定量。
