抽动秽语综合征患者血小板储存颗粒对多巴胺的摄取减少。

Decreased dopamine uptake into platelet storage granules in Gilles de la Tourette disease.

作者信息

Rabey J M, Oberman Z, Graff E, Korczyn A D

机构信息

Department of Neurology, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Israel.

出版信息

Biol Psychiatry. 1995 Jul 15;38(2):112-5. doi: 10.1016/0006-3223(94)00234-T.

DOI:10.1016/0006-3223(94)00234-T

PMID:7578642

Abstract

The movement disorder of Gilles de la Tourette (GdlT) disease may reflect hyperactivity of the basal ganglionic dopamine system. Since platelets have been suggested as peripheral models for the study of catecholamine neurons, we developed a method to measure the uptake of [3H]-DA into platelet storage granules (PSG). In the present report, PSG were incubated with [3H] DA, and Vmax and Km values were calculated by linear regression analysis (Lineweaver Burke plot). The uptake of DA (0.5-5 microM) by PSG from 18 GdlT patients was significantly lower (p < .0001) compared to 15 controls (Vmax mean +/- SD, 107.5 +/- 42.5 and 265.3 +/- 66.5 fmole/mg protein resp.). The decrease of DA uptake in GdlT may reflect compensatory presynaptic changes that reduce DA activity.

摘要

吉勒·德·拉·图雷特综合征（GdlT）的运动障碍可能反映了基底神经节多巴胺系统的功能亢进。由于血小板已被视为研究儿茶酚胺神经元的外周模型，我们开发了一种测量[3H] -多巴胺进入血小板储存颗粒（PSG）摄取量的方法。在本报告中，将PSG与[3H]多巴胺一起孵育，并通过线性回归分析（Lineweaver Burke图）计算Vmax和Km值。与15名对照组相比，18名GdlT患者的PSG对多巴胺（0.5 - 5微摩尔）的摄取明显更低（p <.0001）（Vmax平均值±标准差分别为107.5±42.5和265.3±66.5飞摩尔/毫克蛋白质）。GdlT中多巴胺摄取的减少可能反映了减少多巴胺活性的突触前代偿性变化。