Monfort S L, Harvey E, Geurts L, Padilla L, Simmons H A, Williamson L R, Wildt D E
National Zoological Park, Conservation and Research Center, Smithsonian Institution, Front Royal, Virginia 22630, USA.
Biol Reprod. 1995 Sep;53(3):700-6. doi: 10.1095/biolreprod53.3.700.
To determine the primary excretory by-products of testosterone (T), 85 microCi [3H]T was administered i.v. to two adult Eld's deer stags. Blood (10 ml) was collected by jugular venipuncture at 0, 5, 10, 15, 30, 45, 60, 90, 120, 150, 180, 240, and 480 min after isotope infusion, and all urine and feces were collected for 96 h after injection. Seventy percent of labeled circulating steroid was conjugated by 30 min postinfusion. The majority (80.4 +/- 3.2%) of T metabolites were excreted into urine, and 95.0 +/- 0.9% of these were conjugated, 95.8 +/- 0.2% being hydrolyzable with glucuronidase. Seven urinary androgen metabolites, including androstanediol (5 alpha-androstan-3 alpha-17 beta-diol and 5 beta-androstan-3 alpha-17 beta-diol), were identified in glucoronidase-hydrolyzed, ether-extracted Eld's deer urine pools after gas chromatography/mass spectrometry. A double-antibody 125I RIA for 5 alpha-androstanediol-3 alpha, 17 beta-diol,17-glucuronide (3 alpha-diol-G) was validated for unprocessed urine. Longitudinal assessments of urine samples collected from 13 stages for 3 yr revealed biological concordance between fluctuations in urinary 3 alpha-diol-G and serum T, as well as seasonal changes in secondary sexual characteristics. Overall correlation between "same-day" matched serum T and urinary 3 alpha-diol-G was 0.58, (n = 6; p < 0.001). Thus, monitoring urinary 3 alpha-diol-G provides a noninvasive, alternative method for characterizing male endocrine interrelationships in an endangered ungulate species.
为确定睾酮（T）的主要排泄副产物，对两只成年坡鹿雄鹿静脉注射85微居里的[3H]T。同位素注入后0、5、10、15、30、45、60、90、120、150、180、240和480分钟时，通过颈静脉穿刺采集10毫升血液，注射后96小时收集所有尿液和粪便。注入同位素后30分钟，70%的标记循环类固醇被结合。大部分（80.4±3.2%）T代谢产物排泄到尿液中，其中95.0±0.9%被结合，95.8±0.2%可被葡萄糖醛酸酶水解。气相色谱/质谱分析后，在葡萄糖醛酸酶水解、乙醚萃取的坡鹿尿液样本中鉴定出七种尿雄激素代谢产物，包括雄甾二醇（5α-雄甾烷-3α-17β-二醇和5β-雄甾烷-3α-17β-二醇）。针对5α-雄甾二醇-3α，17β-二醇，17-葡萄糖醛酸苷（3α-二醇-G）的双抗体125I放射免疫分析方法在未处理尿液中得到验证。对13个阶段的尿液样本进行了为期3年的纵向评估，结果显示尿3α-二醇-G波动与血清T之间存在生物学一致性，以及第二性征的季节性变化。“同日”匹配的血清T与尿3α-二醇-G之间的总体相关性为0.58，（n = 6；p < 0.001）。因此，监测尿3α-二醇-G为一种濒危有蹄类物种的雄性内分泌相互关系特征提供了一种非侵入性的替代方法。
