Bordoni A, Hrelia S, Caboni M F, Lercker G, Biagi P L
Department of Biochemistry G Moruzzi, University of Bologna, Italy.
Cardioscience. 1995 Jun;6(2):107-13.
We have investigated the incorporation of cholesterol oxidation products into cardiomyocyte lipids and related this to changes in cell proliferation, evaluated by measuring cellular protein content. Primary cultures of neonatal rat ventricular cells were supplemented with scalar concentrations of several cholesterol oxidation products (cholestan-5 alpha, 6 alpha-epoxy-3 beta-ol, 5 alpha-cholestane-3 beta, 5, 6 beta-triol, 5-cholesten-3 beta, 4 beta-diol, 5-cholesten-3 beta-ol-7-one, and 5-cholesten-3-one). Although all the cholesterol oxidation products were incorporated into the cardiomyocyte lipids when added to the medium at a concentration higher than 0.5 microM, the extent of the incorporation of the different cholesterol oxidation products differed, depending on the concentration in the culture medium and on the chemical structure of the compound. The effects of the cholesterol oxidation products on the cellular protein content were also different: 5 alpha-cholestane-3 beta, 5, 6 beta-triol was shown to be the most potent inhibitor of cell proliferation, followed by cholestan-5 alpha, 6 alpha-epoxy-3 beta-ol, 5-cholesten-3 beta, 4 beta-diol and 5-cholesten-3 beta-ol-7-one. 5-Cholesten-3-one did not affect the cellular protein content. The ability of cholesterol oxidation products to inhibit cell proliferation, and their capacity to increase the permeability of the plasma membrane to calcium, could be deleterious for cardiac cells.
我们研究了胆固醇氧化产物掺入心肌细胞脂质的情况，并将其与通过测量细胞蛋白质含量评估的细胞增殖变化相关联。新生大鼠心室细胞的原代培养物中添加了几种胆固醇氧化产物（胆甾烷-5α，6α-环氧-3β-醇、5α-胆甾烷-3β，5，6β-三醇、5-胆甾烯-3β，4β-二醇、5-胆甾烯-3β-醇-7-酮和5-胆甾烯-3-酮）的标量浓度。尽管当以高于0.5微摩尔的浓度添加到培养基中时，所有胆固醇氧化产物都掺入了心肌细胞脂质中，但不同胆固醇氧化产物的掺入程度有所不同，这取决于培养基中的浓度和化合物的化学结构。胆固醇氧化产物对细胞蛋白质含量的影响也不同：5α-胆甾烷-3β，5，6β-三醇被证明是最有效的细胞增殖抑制剂，其次是胆甾烷-5α，6α-环氧-3β-醇、5-胆甾烯-3β，4β-二醇和5-胆甾烯-3β-醇-7-酮。5-胆甾烯-3-酮不影响细胞蛋白质含量。胆固醇氧化产物抑制细胞增殖的能力及其增加质膜对钙通透性的能力，可能对心脏细胞有害。
