The role of the thymus during T-lymphocyte development in vitro.

To date, fetal thymic organ culture is the only in-vitro system capable of supporting a complete programme of T-lymphocyte development in a manner comparable to that seen in vivo. In this review, we will summarise recent studies in which thymic organ cultures have been used to investigate the regulatory mechanisms of particular stages of thymocyte development. In addition, the use of other culture systems of T-cell maturation will be discussed in an attempt to define the optimal conditions for T-cell development in vitro.