Müller K M, Luedecker C J, Udey M C, Farr A G
Department of Biological Structure, School of Medicine, University of Washington, Seattle 98195, USA.
Immunity. 1997 Mar;6(3):257-64. doi: 10.1016/s1074-7613(00)80328-3.
We examined the role of E-cadherin expressed by thymic epithelial cells and immature thymocytes in thymus organogenesis and thymocyte development. ECCD-1 MAb, which blocks homotypic E-cadherin interactions, inhibited epithelial organization and thymocyte development in reaggregate fetal thymic organ cultures. It also interfered with the differentiation of fetal liver cells or fetal thymocytes within deoxyguanosine-treated thymic lobes, but did not affect thymocyte development in intact cultured fetal thymic lobes. In contrast, antibodies that selectively inhibit interactions between E-cadherin and alpha(E)beta7 integrins did not perturb thymic epithelial organization or thymocyte development, suggesting that homotypic E-cadherin interactions play an important role in thymus development and function.
我们研究了胸腺上皮细胞和未成熟胸腺细胞所表达的E-钙黏蛋白在胸腺器官发生和胸腺细胞发育中的作用。阻断同型E-钙黏蛋白相互作用的ECCD-1单克隆抗体，在重组胎儿胸腺器官培养物中抑制上皮组织形成和胸腺细胞发育。它还干扰了脱氧鸟苷处理的胸腺叶内胎儿肝细胞或胎儿胸腺细胞的分化，但不影响完整培养的胎儿胸腺叶中的胸腺细胞发育。相比之下，选择性抑制E-钙黏蛋白与α(E)β7整合素之间相互作用的抗体不会扰乱胸腺上皮组织或胸腺细胞发育，这表明同型E-钙黏蛋白相互作用在胸腺发育和功能中起重要作用。
