Iglehart J D, Kerns B J, Huper G, Marks J R
Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA.
Breast Cancer Res Treat. 1995 Jun;34(3):253-63. doi: 10.1007/BF00689717.
Ductal carcinoma in situ (intraductal carcinoma) of the breast is a commonly recognized and curable clinical entity. Patients with intraductal carcinoma are at risk to develop invasive breast cancer presumably due to a transition from the noninvasive to the invasive phase of growth. Primary breast malignancies commonly display both in situ and invasive phases of growth in the same tumor. In the current study, DNA content and alterations in the erbB-2 (HER-2/neu) oncogene product were examined simultaneously in both growth phases of primary breast cancers by image analysis. DNA content in the intraductal and invasive components of primary breast cancers were virtually identical (r = 0.979, p < 0.001). Quantitative image analysis was used to measure erbB-2 expression and categories of expression were related to copy number of the erbB-2 gene. Expression of erbB-2 was similar in both growth phases and implies identity of the erbB-2 genotype. The identity of DNA content suggests that the noninvasive and invasive phases within a single breast cancer are highly related. It is likely that erbB-2 gene number remains the same during progression from intraductal to invasive disease.
乳腺导管原位癌（导管内癌）是一种公认的可治愈的临床实体。导管内癌患者有发展为浸润性乳腺癌的风险，推测是由于从非浸润性生长阶段转变为浸润性生长阶段所致。原发性乳腺恶性肿瘤通常在同一肿瘤中同时表现出原位生长和浸润性生长阶段。在本研究中，通过图像分析同时检测了原发性乳腺癌两个生长阶段的DNA含量和erbB-2（HER-2/neu）癌基因产物的改变。原发性乳腺癌导管内成分和浸润性成分的DNA含量几乎相同（r = 0.979，p < 0.001）。采用定量图像分析来测量erbB-2表达，表达类别与erbB-2基因拷贝数相关。erbB-2在两个生长阶段的表达相似，这意味着erbB-2基因型相同。DNA含量相同表明单个乳腺癌内的非浸润性和浸润性阶段高度相关。从导管内癌发展到浸润性疾病的过程中，erbB-2基因数量可能保持不变。
