鸡胚Wnt-4和Wnt-11 cDNA的克隆与鉴定

Cloning and characterization of Wnt-4 and Wnt-11 cDNAs from chick embryo.

作者信息

Tanda N, Kawakami Y, Saito T, Noji S, Nohno T

机构信息

Department of Pharmacology, Kawasaki Medical School, Okayama, Japan.

出版信息

DNA Seq. 1995;5(5):277-81. doi: 10.3109/10425179509030981.

DOI:10.3109/10425179509030981

PMID:7579581

Abstract

We have isolated two members of the Wnt gene family, Wnt-4 and Wnt-11, from chick embryo cDNA library, and determined the entire coding sequences. The Wnt-4 and Wnt-11 genes encode secretory proteins composed of 351 and 354 amino acids, respectively, both having 24 Cys residues conserved among other Wnt family members. Alignment of the deduced amino acid sequences reveals that chicken Wnt-4 and Wnt-11 are most similar to Xenopus Wnt-4 and mouse Wnt-11; respectively. Northern blot analysis indicates the Wnt-4 expression at 1.5 kilobase and the Wnt-11 expression at 2.0 kilobase in the chick embryo.

摘要

我们从鸡胚cDNA文库中分离出Wnt基因家族的两个成员Wnt - 4和Wnt - 11，并确定了其完整编码序列。Wnt - 4和Wnt - 11基因分别编码由351和354个氨基酸组成的分泌蛋白，两者都有24个在其他Wnt家族成员中保守的半胱氨酸残基。推导的氨基酸序列比对显示，鸡的Wnt - 4和Wnt - 11分别与非洲爪蟾的Wnt - 4和小鼠的Wnt - 11最为相似。Northern印迹分析表明，在鸡胚中Wnt - 4在1.5千碱基处表达，Wnt - 11在2.0千碱基处表达。