Tanda N, Ohuchi H, Yoshioka H, Noji S, Nohno T
Department of Pharmacology, Kawasaki Medical School, Kurashiki City, Japan.
Biochem Biophys Res Commun. 1995 Jun 6;211(1):123-9. doi: 10.1006/bbrc.1995.1786.
We have isolated a new member of the Wnt gene family, Wnt-11, from chick embryo cDNA library and examined the expression pattern during embryogenesis by in situ hybridization. The Wnt-11 gene encodes Cys-rich secretory protein distantly related to Wnt-1 through Wnt-8 from the mouse and Xenopus. Expression of the Wnt-11 gene became evident at stage 14 in the dorsolateral region of somites and gradually restricted to the dermatome at stage 19 and later. In contrast to the other Wnt genes, Wnt-11 was not expressed in the neuroepithelium throughout stages 14-26. At stage 24 and later, Wnt-11 was expressed in the subectodermal mesenchyme of the limb and feather buds. The unique expression pattern of Wnt-11 in the paraxial mesoderm and dermatome suggests that Wnt-11 may play an important role in dermal development.
我们从鸡胚cDNA文库中分离出Wnt基因家族的一个新成员Wnt-11，并通过原位杂交检测了其在胚胎发生过程中的表达模式。Wnt-11基因编码一种富含半胱氨酸的分泌蛋白，与小鼠和非洲爪蟾的Wnt-1至Wnt-8有较远的亲缘关系。Wnt-11基因的表达在第14阶段体节的背外侧区域变得明显，并在第19阶段及以后逐渐局限于生皮节。与其他Wnt基因不同，Wnt-11在第14至26阶段的神经上皮中均未表达。在第24阶段及以后，Wnt-11在肢体和羽毛芽的外胚层下间充质中表达。Wnt-11在轴旁中胚层和生皮节中的独特表达模式表明，Wnt-11可能在皮肤发育中起重要作用。
