Kane J F
SmithKline Beecham Pharmaceuticals, King of Prussia, USA.
Curr Opin Biotechnol. 1995 Oct;6(5):494-500. doi: 10.1016/0958-1669(95)80082-4.
Within Escherichia coli and other species, a clear codon bias exists among the 61 amino acid codons found within the population of mRNA molecules, and the level of cognate tRNA appears directly proportional to the frequency of codon usage. Given this situation, one would predict translational problems with an abundant mRNA species containing an excess of rare low tRNA codons. Such a situation might arise after the initiation of transcription of a cloned heterologous gene in the E. coli host. Recent studies suggest clusters of AGG/AGA, CUA, AUA, CGA or CCC codons can reduce both the quantity and quality of the synthesized protein. In addition, it is likely that an excess of any of these codons, even without clusters, could create translational problems.
在大肠杆菌和其他物种中，在mRNA分子群体中发现的61种氨基酸密码子之间存在明显的密码子偏好，并且同源tRNA的水平似乎与密码子使用频率成正比。在这种情况下，可以预测，含有过量稀有低tRNA密码子的丰富mRNA种类会出现翻译问题。这种情况可能在大肠杆菌宿主中克隆的异源基因转录开始后出现。最近的研究表明，AGG/AGA、CUA、AUA、CGA或CCC密码子簇会降低合成蛋白质的数量和质量。此外，即使没有密码子簇，这些密码子中的任何一种过量都可能产生翻译问题。
