Neev J, Schiewe M C, Sung V W, Kang D, Hezeleger N, Berns M W, Tadir Y
Beckman Laser Institute, University of California-Irvine 92715, USA.
J Assist Reprod Genet. 1995 Apr;12(4):288-93. doi: 10.1007/BF02212934.
A noncontact holmium:yttrium scandium gallium garnet (Ho:YSGG) laser system has been designed and tested for the micromanipulation of mammalian embryos. The purpose of this preliminary investigation was to determine the effectiveness of this laser for assisted hatching and evaluate its impact on embryo viability. The Ho:YSGG system, utilizing 250-microsecond pulses at a wavelength of 2.1 microns and 4 Hz, was used to remove a portion of the zona pellucida (ZP) of two- to four-cell FVB mouse embryos.
In the first experiment there was no difference in blastocyst production or hatching rates following laser or conventional assisted hatching (LAH or AH, respectively) in contrast to control embryos cultured in a 5% CO2 humidified air incubator at 37 degrees C. In the second experiment a blastocyst antihatching culture model was employed and LAH-treated embryos were cultured in a serum-free HTF medium (HTF-o). Blastocyst formation was not influenced by LAH treatment and hatching was increased (P < 0.01) from 4 to 60% compared to HTF-o control group.
These preliminary data demonstrate the utility and nontoxic properties of the Ho:YSGG laser system for quick and precise ZP drilling.
设计并测试了一种用于哺乳动物胚胎显微操作的非接触钬：钇钪镓石榴石（Ho:YSGG）激光系统。这项初步研究的目的是确定该激光在辅助孵化中的有效性，并评估其对胚胎活力的影响。Ho:YSGG系统利用波长为2.1微米、频率为4赫兹的250微秒脉冲，用于去除二至四细胞期FVB小鼠胚胎的部分透明带（ZP）。
在第一个实验中，与在37摄氏度、5%二氧化碳加湿空气培养箱中培养的对照胚胎相比，激光辅助孵化（LAH）或传统辅助孵化（AH）后的囊胚形成率或孵化率没有差异。在第二个实验中，采用了囊胚抗孵化培养模型，将LAH处理的胚胎在无血清的HTF培养基（HTF-o）中培养。LAH处理对囊胚形成没有影响，与HTF-o对照组相比，孵化率从4%提高到60%（P<0.01）。
这些初步数据证明了Ho:YSGG激光系统在快速精确的ZP钻孔方面的实用性和无毒特性。
