Schiewe M C, Neev J, Hazeleger N L, Balmaceda J P, Berns M W, Tadir Y
Department of Obstetrics and Gynecology, University of California-Irvine, Orange 92668, USA.
Hum Reprod. 1995 Jul;10(7):1821-4. doi: 10.1093/oxfordjournals.humrep.a136183.
The purpose of this study was to assess the efficacy of the holmium:yttrium scandian gallium garnet (Ho:YSGG) laser, operating in a pipette-free, non-contact mode, to assist hatching and sustain normal embryonic development. Two-cell mouse embryos were recovered and assigned to laser-assisted hatching (LAH) treatment or control human tubal fluid (HTF) culture with or without serum (HTF-s, HTF-o) or with late serum supplementation (HTF-o/s). The basic experimental apparatus for LAH consisted of a stationary 2.1 microns Ho:YSGG laser beam directed through a mechanical shutter into an input port of a Zeiss Axiomat inverted microscope. Fewer (P < 0.05) embryos developed to the blastocyst stage in the HTF-s group (81%) than in the LAH (90%), HTF-o (94%) and HTF-o/s (92%) groups. The level of hatching was significantly increased (P < 0.01) after the LAH treatment (57%) compared to HTF-o/s (32%), HTF-s (18%) or HTF-o (5%). Implantation rates were not significantly impaired following the LAH treatment (21%). These data demonstrate that LAH using the Ho:YSGG laser is a simple, accurate and effective procedure for assisted hatching.
本研究的目的是评估以无移液器、非接触模式操作的钬:钇钪镓石榴石（Ho:YSGG）激光辅助孵化并维持正常胚胎发育的效果。回收二细胞期小鼠胚胎，并将其分配至激光辅助孵化（LAH）处理组或置于含或不含血清（HTF-s、HTF-o）或后期补充血清（HTF-o/s）的人输卵管液（HTF）中培养的对照组。LAH的基本实验装置包括一束固定的2.1微米Ho:YSGG激光束，该激光束通过一个机械快门导入蔡司Axiovert倒置显微镜的输入端口。HTF-s组（81%）发育至囊胚期的胚胎数量少于LAH组（90%）、HTF-o组（94%）和HTF-o/s组（92%）（P<0.05）。与HTF-o/s组（32%）、HTF-s组（18%）或HTF-o组（5%）相比，LAH处理后孵化水平显著提高（P<0.01）（57%）。LAH处理后着床率未受到显著影响（21%）。这些数据表明，使用Ho:YSGG激光进行LAH是一种简单、准确且有效的辅助孵化方法。
