Yoshida K, Kondo F
Miyazaki University, Faculty of Agriculture, Department of Veterinary Public Health, Japan.
J AOAC Int. 1995 Jul-Aug;78(4):1126-9.
A rapid, simple, and accurate method for determination of furazolidone (FZ) in swine serum and avian egg using liquid chromatography (LC) with a 358 nm ultraviolet-visible spectrophotometric detector is described. After liquid-liquid extraction of sample with ethyl acetate using Extrelut-3, the extract is evaporated, redissolved in 40% acetonitrile, and injected directly into the chromatograph. The antibiotic can be analyzed within 30 min. Within-day recoveries for swine serum and avian egg spiked with FZ at 1 ppm were 90.0 and 88.1%, respectively, with coefficients of variation of 3.52 and 3.88%, respectively. Between days recoveries for the 1 ppm samples were 87.2 and 87.0%, with coefficients of variation of 3.10 and 4.29%, respectively. Determination of FZ also was performed by LC/mass spectrometry (MS) with an atmospheric-pressure chemical-ionization interface (APCI) system. The LC/MS-APCI system is more applicable for qualitative analysis than quantitative analysis because the drug detection limit (about 0.1 microgram/L) is almost the same as that of the LC-UV detector.
描述了一种使用配备358 nm紫外可见分光光度检测器的液相色谱（LC）快速、简单且准确地测定猪血清和禽蛋中呋喃唑酮（FZ）的方法。使用Extrelut-3以乙酸乙酯对样品进行液液萃取后，将萃取液蒸发，再溶解于40%乙腈中，然后直接注入色谱仪。该抗生素可在30分钟内完成分析。在猪血清和添加了1 ppm FZ的禽蛋中，日内回收率分别为90.0%和88.1%，变异系数分别为3.52%和3.88%。1 ppm样品的日间回收率分别为87.2%和87.0%，变异系数分别为3.10%和4.29%。还采用配备大气压化学电离接口（APCI）系统的液相色谱/质谱（MS）法对FZ进行了测定。液相色谱/质谱-大气压化学电离系统更适用于定性分析而非定量分析，因为药物检测限（约0.1微克/升）与液相色谱-紫外检测器的检测限几乎相同。
