Cieri U R
J Assoc Off Anal Chem. 1979 Jan;62(1):168-70.
The feed sample is extracted with acetone or dimethylformamide-acetone (1 + 1) and the filtered extracts are evaporated to dryness. The residue is dissolved in chloroform and transferred to a silica gel column. The nitrofurans are eluted with methanol-chloroform (50 + 50). A portion of the eluate is evaporated to dryness and the residue is redissolved in a small volume of methanol. Aliquots of the methanolic solution are injected into a liquid chromatograph with a muBondapak C18 column, using 30% acetonitrile as the eluting solvent and ultraviolet detection at 365 nm. Several samples spiked with 0.5--50 ppm furazolidone or nitrofurazone and 2 commercial samples were analyzed by the proposed method.
饲料样品用丙酮或二甲基甲酰胺 - 丙酮（1 + 1）萃取，过滤后的萃取液蒸发至干。残留物溶于氯仿并转移至硅胶柱。用甲醇 - 氯仿（50 + 50）洗脱硝基呋喃。取一部分洗脱液蒸发至干，残留物再溶解于少量甲醇中。取甲醇溶液的等分试样注入配有μBondapak C18柱的液相色谱仪中，以30%乙腈作为洗脱溶剂，并在365nm处进行紫外检测。用所提出的方法分析了几个添加了0.5 - 50ppm呋喃唑酮或呋喃西林的样品以及2个商业样品。
