Feldsine P T, Falbo-Nelson M T
BioControl Systems, Inc., Bothell, WA 98011, USA.
J AOAC Int. 1995 Jul-Aug;78(4):993-7.
A wide variety of naturally contaminated and inoculated raw flesh and highly contaminated food types was analyzed by a modified immunodiffusion enrichment protocol and the Bacteriological Analytical Manual (BAM) method to determine the equivalence of these methods. This modification was developed by Agriculture Canada to allow addition of a high-temperature selective enrichment step in tetrathionate brilliant green broth at 42 degrees C while maintaining a 2-day total test time. Foods representing red meat, white meat, frog, and seafoods and one type of animal meal were evaluated. A total of 320 samples was tested, resulting in false negative rates of 5.2 and 3.5%, respectively, for the modified immunodiffusion and the BAM culture methods. The overall agreement rate was 96.9%.
采用改良免疫扩散富集方案和《细菌学分析手册》（BAM）方法，对多种天然污染和接种的生肉以及高度污染的食物类型进行分析，以确定这些方法的等效性。这种改良由加拿大农业部门研发，可在42摄氏度的四硫磺酸钠煌绿肉汤中添加高温选择性富集步骤，同时将总检测时间保持在2天。对代表红肉、白肉、青蛙、海鲜和一种动物饲料的食物进行了评估。共检测了320个样本，改良免疫扩散法和BAM培养法的假阴性率分别为5.2%和3.5%。总体符合率为96.9%。
