Warburton D W, Feldsine P T, Falbo-Nelson M T
Health Canada, Sir Frederick G. Banting Research Center, Ottawa, ON.
J AOAC Int. 1995 Jan-Feb;78(1):59-68.
A total of 19 government and private industry laboratories in Canada and the United States participated in the collaborative study. Naturally contaminated ground poultry and animal meals, as well as inoculated raw shrimp, were examined for presence of Salmonella by both the modified immunodiffusion method and the Bacteriological Analytical Manual culture method, resulting in an agreement rate of 93.1%. The 2 methods are statistically equivalent for all food types at each inoculation level and for all lots of naturally contaminated foods evaluated in this study. The modification of the AOAC Official Method 989.13, immunodiffusion (1-2 TEST) method for detection of motile Salmonella in all foods, has been adopted revised first action by AOAC INTERNATIONAL.
加拿大和美国共有19个政府及私营行业实验室参与了这项合作研究。采用改良免疫扩散法和《细菌学分析手册》培养法，对天然污染的地面家禽和动物饲料以及接种的生虾进行沙门氏菌检测，一致率为93.1%。在本研究评估的每个接种水平下，对于所有食品类型以及所有批次的天然污染食品，这两种方法在统计学上是等效的。AOAC国际组织已采用对AOAC官方方法989.13（免疫扩散（1-2 TEST）法）进行的修改，用于检测所有食品中的运动型沙门氏菌，该修改已被AOAC国际组织采纳为首次修订行动。
