[Elaboration of the polymerase chain reaction system for subtype specific differentiation of influenza A virus strains].
作者信息
Tarasov K V, Tsvirkunova S Iu, Bekhtereva T A
出版信息
Vestn Ross Akad Med Nauk. 1995(9):22-4.
PMID:7580407
Abstract
New subtype specific primers for hemagglutinin (H1, H2, H3) of influenza A viruses were selected. The specificity of the primers' performance was tested in a model experiment which used cDNA synthesized on the vRNA from the collection of influenza A viruses (H1, H2, H3 subtypes) as templates. The sensibility of the PCR system was determined on DNA plasmids- and RNA templates (with preliminary reverse transcription) and amounted to 10(2) and 10(5)-10(6) virion particles, respectively.
摘要
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