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[甲型H1N1流感病毒血凝素基因全长DNA拷贝的合成、克隆及一级结构]

[Synthesis of a full-length DNA copy of the hemagglutinin gene of the the influenza virus A H1N1 subtype, its cloning and primary structure].

作者信息

Beklemishev A B, Blinov V M, Vasilenko S K, Golovin S Ia, Gutorov V V

出版信息

Bioorg Khim. 1984 Nov;10(11):1535-43.

PMID:6098280
Abstract

Full-length DNA-copy of hemagglutinin gene RNA of influenza A virus strain A/Leningrad/54/1 was synthesized and cloned in E. coli plasmid pBR 327. Its primary structure was determined by a modified Maxam - Gilbert procedure. The nucleotide sequence of this gene was compared with sequences of analogous genes of influenza strains A/USSR/90/77 and A/PR/8/34. An addition of one nucleotide in non-translated region was found in the former.

摘要

合成了甲型流感病毒A/列宁格勒/54/1株血凝素基因RNA的全长DNA拷贝,并将其克隆到大肠杆菌质粒pBR 327中。通过改良的马克萨姆-吉尔伯特方法确定了其一级结构。将该基因的核苷酸序列与甲型流感病毒A/苏联/90/77株和A/PR/8/34株的类似基因序列进行了比较。发现前者在非翻译区多了一个核苷酸。

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