Beklemishev A B, Blinov V M, Vasilenko S K, Golovin S Ia, Gutorov V V
Bioorg Khim. 1984 Nov;10(11):1535-43.
Full-length DNA-copy of hemagglutinin gene RNA of influenza A virus strain A/Leningrad/54/1 was synthesized and cloned in E. coli plasmid pBR 327. Its primary structure was determined by a modified Maxam - Gilbert procedure. The nucleotide sequence of this gene was compared with sequences of analogous genes of influenza strains A/USSR/90/77 and A/PR/8/34. An addition of one nucleotide in non-translated region was found in the former.
合成了甲型流感病毒A/列宁格勒/54/1株血凝素基因RNA的全长DNA拷贝,并将其克隆到大肠杆菌质粒pBR 327中。通过改良的马克萨姆-吉尔伯特方法确定了其一级结构。将该基因的核苷酸序列与甲型流感病毒A/苏联/90/77株和A/PR/8/34株的类似基因序列进行了比较。发现前者在非翻译区多了一个核苷酸。