Landureau J C, Achour A, Fouchard M, M'Bika J P, Mazière J C, Zagury D
Laboratoire de Physiologie Cellulaire, Université Pierre et Marie Curie, Paris, France.
Cell Mol Biol (Noisy-le-grand). 1995 May;41(3):423-30.
Under standard conditions, all established cell-lines of HIV-1-infected lymphocytes are cultured in a medium containing a high percentage of serum. We have found that whilst partial or total serum depletion impedes their cell growth, it dramatically increases their virus production. Serial subcultures in serum-depleted medium are necessary to insure permanent cell growth and a relatively high reverse transcriptase activity. Following optimization of culture conditions, both HIV-infected and uninfected H9 cells have been regularly grown in a new protein-free medium. Such chemically defined culture conditions were required to detect the effect of minor components, such as vitamins, on host cell-virus interactions.
在标准条件下，所有已建立的感染HIV-1的淋巴细胞系都在含有高比例血清的培养基中培养。我们发现，虽然部分或完全去除血清会阻碍其细胞生长，但会显著增加其病毒产生量。为确保细胞持续生长和相对较高的逆转录酶活性，需要在无血清培养基中进行连续传代培养。优化培养条件后，已感染和未感染HIV的H9细胞都能在一种新的无蛋白培养基中正常生长。需要这种化学成分明确的培养条件来检测维生素等微量成分对宿主细胞与病毒相互作用的影响。
